Vertical profiles of the cloud particle phase state in tropical deep-convective clouds (DCCs) were investigated using airborne solar radiation data collected by the German research aircraft HALO during the ACRIDICON-CHUVA campaign, which was conducted over the Brazilian Amazon in September 2014. A phase discrimination retrieval based on imaging spec-troradiometer measurements of cloud side spectral reflectivity was applied to DCCs under different aerosol conditions. From the retrieval results the height of the mixed phase layer of the DCCs was determined. The retrieved profiles were compared with in situ measurements and satellite observations. It was found that the depth and vertical position of the mixed phase layer can vary up to 900 m for one single cloud scene. In particular, this variation is attributed to the different stages of cloud development in one scene. Clouds of mature or decaying stage are affected by falling ice particles resulting in lower levels of fully glaciated cloud layers compared to growing clouds. Comparing polluted and moderate aerosol conditions revealed a shift of the lower boundary of the mixed phase layer from 5.6 ± 0.2 km (269 K) [moderate] to 6.2 ± 0.3 km (267 K) [polluted], and of the upper boundary from 6.8 ± 0.2 km (263 K) [moderate] to 7.4 ± 0.4 km (259 K) [polluted], as would be expected from theory.