A new monoterpenoid indole alkaloid, 10-methoxyakuammidine ( 1 ), together with four known alkaloids ( 2–5 ), were isolated from the stems and leaves of Ochrosia elliptica . The structure of 1 was elucidated by extensive spectroscopic methods and the known compounds were identified by comparisons with data reported in the literature. New compound 1 was evaluated for its cytotoxicities against five human cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7 and SW480 in vitro . 1 exhibited inhibitory effects with IC 50 values comparable to those of cisplatin.