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Oxford University Press, The Plant Cell, 4(24), p. 1316-1326, 2012

DOI: 10.1105/tpc.112.096362

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Secretory Pathway Research: The More Experimental Systems the Better

Journal article published in 2012 by Jurgen Denecke, Fernando Aniento ORCID, Lorenzo Frigerio ORCID, Chris Hawes, Inhwan Hwang, Jaideep Mathur, Jean-Marc Neuhaus, David G. Robinson
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopic expression and the use of dominant mutants can provide insights into protein function.