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American Society for Microbiology, Journal of Virology, 4(73), p. 3473-3476, 1999

DOI: 10.1128/jvi.73.4.3473-3476.1999

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Direct Evidence that the Poly(A) Tail of Influenza A Virus mRNA Is Synthesized by Reiterative Copying of a U Track in the Virion RNA Template

Journal article published in 1999 by Leo L. M. Poon ORCID, David C. Pritlove, Ervin Fodor ORCID, George Gg Brownlee
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT The poly(A) tail of influenza virus mRNA is thought to be synthesized by reiterative copying of the U track near the 5′ end of the virion RNA template. This has been widely accepted as a plausible hypothesis, but until now there has been no direct experimental evidence for it. Here, we report such direct evidence based on the fact that (i) replacing the U track with an A track directs synthesis of products with poly(U) tails, both in vitro and in vivo, and (ii) interrupting the U track abolishes polyadenylation in vitro.