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Wiley Open Access, FASEB Journal, S1(26), 2012

DOI: 10.1096/fasebj.26.1_supplement.941.3

Oxford University Press, Nucleic Acids Research, 10(40), p. 4701-4710, 2012

DOI: 10.1093/nar/gks032

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Transgene regulation in plants by alternative splicing of a suicide exon

Journal article published in 2012 by Scott F. Hickey, Malathy Sridhar, Alexander J. Westermann ORCID, Ming Chen Hammond, Qian Qin, Pooja Vijayendra, Geoffrey Liou
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Compared to transcriptional activation, other mechanisms of gene regulation have not been widely exploited for the control of transgenes. One barrier to the general use and application of alternative splicing is that splicing-regulated transgenes have not been shown to be reliably and simply designed. Here, we demonstrate that a cassette bearing a suicide exon can be inserted into a variety of open reading frames (ORFs), generating transgenes whose expression is activated by exon skipping in response to a specific protein inducer. The surprisingly minimal sequence requirements for the maintenance of splicing fidelity and regulation indicate that this splicing cassette can be used to regulate any ORF containing one of the amino acids Glu, Gln or Lys. Furthermore, a single copy of the splicing cassette was optimized by rational design to confer robust gene activation with no background expression in plants. Thus, conditional splicing has the potential to be generally useful for transgene regulation.