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Elsevier, Molecular and Cellular Proteomics, 11(12), p. 3026-3035, 2013

DOI: 10.1074/mcp.o113.029777

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Tools (Viewer, Library and Validator) that Facilitate Use of the Peptide and Protein Identification Standard Format, Termed mzIdentML*

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Data provided by SHERPA/RoMEO

Abstract

The Proteomics Standards Initiative has recently released the mzIdentML data standard for representing peptide and protein identification results, for example created by a search engine. When a new standard format is produced, it is important that software tools are available that make it straightforward for laboratory scientists to use it routinely, and for bioinformaticians to embed support in their own tools. Here we report the release of several open source Java-based software packages, based around mzIdentML: ProteoIDViewer, mzidLibrary and mzidValidator. The ProteoIDViewer is a desktop application allowing users to visualise mzIdentML-formatted results originating from any appropriate identification software, supporting visualisation of all the features of the mzIdentML format. The mzidLibrary is a software library, containing routines for importing data from external search engines, post-processing identification data (such as false discovery rate calculations), combining results from multiple search engines, performing protein inference, setting identification thresholds and exporting results from mzIdentML to plain text files. The mzidValidator is able to process files and report warnings or errors if files are not correctly formatted or contain some semantic error. We anticipate that these developments will simplify adoption of the new standard in proteomics laboratories and integration of mzIdentML into other software tools. All three tools are freely available in the public domain from: http://code.google.com/p/mzidentml-viewer/ (ProteoIDViewer), http://code.google.com/p/mzidentml-lib/ (mzidLibrary) and http://code.google.com/p/psi-pi/downloads/list (mzidValidator).