Published in
Nature Research, Nature Communications, 1(6), 2015
DOI: 10.1038/ncomms7178
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- ORCID
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- [www.ncbi.nlm.nih.gov] | PDF
- [kclpure.kcl.ac.uk] | PDF
- [kclpure.kcl.ac.uk] | PDF
- [europepmc.org] | PDF
- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
Tools
Capture Hi-C identifies the chromatin interactome of colorectal cancer risk loci
,
Radhika Kandaswamy ,
Helen E. Speedy,
Andreas Heindl,
Nicola Whiffin,
Maria J. Carnicer,
Laura Broome,
Nicola Dryden,
Takashi Nagano,
Stefan Schoenfelder,
Martin Enge,
Yinyin Yuan,
Jussi Taipale
and other authors.
Full text: Download
Abstract
AbstractMultiple regulatory elements distant from their targets on the linear genome can influence the expression of a single gene through chromatin looping. Chromosome conformation capture implemented in Hi-C allows for genome-wide agnostic characterization of chromatin contacts. However, detection of functional enhancer–promoter interactions is precluded by its effective resolution that is determined by both restriction fragmentation and sensitivity of the experiment. Here we develop a capture Hi-C (cHi-C) approach to allow an agnostic characterization of these physical interactions on a genome-wide scale. Single-nucleotide polymorphisms associated with complex diseases often reside within regulatory elements and exert effects through long-range regulation of gene expression. Applying this cHi-C approach to 14 colorectal cancer risk loci allows us to identify key long-range chromatin interactions in cis and trans involving these loci.