Humana Press, Methods in Molecular Biology, p. 1-20, 2021
DOI: 10.1007/978-1-0716-1024-4_1
Humana Press, Methods in Molecular Biology, p. 3-21, 2012
DOI: 10.1007/978-1-61779-885-6_1
Full text: Unavailable
AbstractMass spectrometry is frequently used in quantitative proteomics to detect differentially regulated proteins. A very important but unfortunately oftentimes neglected part in detecting differential proteins is the statistical analysis. Data from proteomics experiments are usually high-dimensional and hence require profound statistical methods. It is especially important to already correctly design a proteomic experiment before it is conducted in the laboratory. Only this can ensure that the statistical analysis is capable of detecting truly differential proteins afterward. This chapter thus covers aspects of both statistical planning as well as the actual analysis of quantitative proteomic experiments.