Coincident multichannel seismic reflection and refraction data from a N-S transect near Oahu, Hawaii, provide evidence for thickening of the Pacific crust by 1-2+/-1 km south of the large-offset (16 m.y.) Molokai Fracture Zone (FZ). Tau-p stacks, tau-sum inversions, and forward modeling of the refraction data indicate that the crustal thickening occurs primarily within the lower portion of seismic layer 2. Assuming isostatic balance, the differences in crustal thickness predict that seafloor having the same age will have different elevations across the FZ. Observations of seafloor depths across the FZ east of the Hawaiian Islands are consistent with this prediction implying that the processes which have generated the crustal differences have been stable for over 50 m.y. Previous correlations between the chemical composition of ridge crest, basalts, crustal thickness, and ridge crest elevation have been attributed to variations in the thermal regime of the upper mantle under mid-ocean spreading centers. In accord with this hypothesis, we propose that the observed differences in crustal structure across the Molokai FZ may have been produced by small (25°C) differences in the thermal regime of the upper mantle beneath the ancestral East Pacific Rise. Discontinuous intracrustal reflections located about 1.6 s below the sediment/basement interface are observed in migrated reflection data south of Oahu. These reflections are similar in character to the lower crustal ``Horizon R'' event observed in the western North Atlantic. Shallower intracrustal reflections, possibly from within seismic layer 2, are also observed. The observation of these intracrustal reflections in both the Atlantic and Pacific oceans suggests that they are a fundamental signature of the crustal accretion process at a variety of spreading rates and that they are mappable using modern seismic reflection/refraction methods.