Frequent hypermethylation of a representative conserved intergenic region. A, DNA methylation signals (smoothed adjusted log2(M/T)) surrounding a representative intergenic region that was identified to be hypermethylated in the LNCaP cells compared to PrEC cells. The shown region is annotated with the chromosome coordinates (top), CpG density (number of CpGs in sliding 250 bp windows), and PhastCons scores. The boxed area represents the region identified to be hypermethylated. Note that the region overlaps sequences with high conservation as indicated by high PhastCons scores. B, A waterfall plot of the extent of hypermethylation of the boxed region from panel (A) in paired tumor-normal prostate tissues.