Comparison of mapping mismatches. Percentage of reads with different numbers of mismatches in the mapping between the reads produced by either HPCall or the native 454 base-caller and the E. coli K-12 reference sequence. For mapping either ssaha2 or subread is used. Detected number of sequence variants for the E. coli data set using ssahaSNP. HPCall results in more perfect-matching reads and less overall indels and SNPs.