Depth of coverage for each amplicon pool derived from first cohort sequencing data. Blue line depicts absolute coverage for plus-strand aligned reads. Green line depicts coverage of minus-strand aligned reads. Scales of X- and Y-axes are identical for all graphs depicted for each exon. Light red line indicates presumptive mismatch rate determined from plus-strand aligned reads. Light orange line indicates presumptive mismatch rate determined from minus-strand aligned reads. Ratio of mismatch rate between plus and minus strands is later incorporate into the tailcurve factor used in filtering by SERVIC4E.