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American Society for Microbiology, Journal of Clinical Microbiology, 4(47), p. 1238-1240, 2009

DOI: 10.1128/jcm.02204-08

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Single-Point Mutations Causing More than 100-Fold Underestimation of Human Immunodeficiency Virus Type 1 (HIV-1) Load with the Cobas TaqMan HIV-1 Real-Time PCR Assay

Journal article published in 2009 by Klaus Korn ORCID, Benedikt Weissbrich, Cornelia Henke-Gendo, Albert Heim ORCID, Christin Mariel Jauer, Ninon Taylor, Josef Eberle
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 M gag sequences.