Cotton seedling damping-off is considered a disease complex, in which several pathogens can be involved. In SW Spain, postemergence damping-off seems to be mainly associated with Rhizoctonia solani and Thielaviopsis basicola, posing a serious limitation for crop, especially in cold springs. Ninety-seven commercial plots, where postemergence damping-off of cotton seedlings was observed during previous years, were selected in April 2001. In each plot, plants were randomly sampled between cotyledon to three true-leaf stage and soil samples besides the plants were taken. Symptomatic plants were separated according to the main observable seedling disease symptom: black necrosis (black root rot), brown necrosis and other symptoms. Thielaviopsis basicola inoculum was estimated in soil samples. Soil samples were also analysed for nutrient availability (N, P, K, Ca, Mg, Fe, Cu, Mn and Zn). All the sampled plants showed some seedling disease symptom. Macroscopic symptoms can provide a reasonable distinction between these two major pathogens involved in seedling disease symptoms in the studied area: the percentage of T. basicola isolates (18%) from black necrosis symptomatic plants was significantly higher than that of R. solani (4.1%), whereas in brown necrosis symptomatic plants, the situation was reversed (10.7 vs. 12.8%). The percentage of plants with black necrosis symptoms was inversely related to the portion of plants with brown necrosis in each plot. The mean incidence of black necrosis was significantly lower in plots with residue incorporation (sugar beet as the preceding crop) than in plots without residue incorporation. No significant effect of preceding crop or residue management on brown necrosis incidence was observed. Incidence of black necrosis was negatively correlated with available N measured as NO3-N when corn or sunflower were the preceding crop. The incidence of black necrosis was positively related to Fe availability in soil after cotton as preceding crop, whereas brown necrosis was negatively related to the availability of this micronutrient.