Published in

The Company of Biologists, Biology Open, 4(2), p. 416-423, 2013

DOI: 10.1242/bio.20133780

Links

Tools

Export citation

Search in Google Scholar

FAK–Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique

Journal article published in 2013 by Guangping Tai, Peter Hohenstein ORCID, Jamie A. Davies
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Green circle
Published version: archiving allowed
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

Summary This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The final stage is ex vivo organ culture. We demonstrate the utility of the screen by using it to identify the FAK–Src-pathway signalling as being important for collecting duct development, specifically for the cell proliferation on which this development depends.