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Springer, Photochemical & Photobiological Sciences, 2(12), p. 231-235, 2013

DOI: 10.1039/c2pp25254f

Elsevier, Biophysical Journal, 2(104), p. 672a, 2013

DOI: 10.1016/j.bpj.2012.11.3713

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A Photochromic Bacterial Photoreceptor with Potential for Super-Resolution Microscopy

Journal article published in 2013 by Aba Losi ORCID, Francesca Cella Zanacchi, Wolfgang Gärtner ORCID, Sarah Raffelberg, Francesca Cella Zanacchi ORCID, Paolo Bianchini, Alberto Diaspro, Carmen Mandalari, Stefania Abbruzzetti, Cristiano Viappiani
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We introduce a novel fluorescent reporter with potential for super-resolution microscopy, based on the bacterial photoreceptor YtvA. YtvA (from Bacillus subtilis) comprises a photosensitive flavin-based LOV domain, efficiently photo-switchable between fluorescent and non-fluorescent states. We demonstrate Fluorescence PhotoActivation Localization Microscopy (FPALM) studies of live Escherichia coli cells, expressing YtvA molecules.