Published in
Elsevier, Journal of Microbiological Methods, 3(91), p. 358-360, 2012
DOI: 10.1016/j.mimet.2012.09.007
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Production and purification of theVP1 capsid protein of a novel canine norovirus using the Saccharomyces cerevisiae expression system
Journal article published in 2012 by
Clara Pereira 
,
M. São José Nascimento,
Edite Lopes,
João R. Mesquita,
M. São José Nascimento,
Lucília Saraiva
,
M. São José Nascimento,
Edite Lopes,
João R. Mesquita,
M. São José Nascimento,
Lucília Saraiva
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Abstract
The discovery of a novel canine norovirus was recently reported. The VP1 capsid protein of this canine norovirus was expressed in Saccharomyces cerevisiae and purified with a yield of 1.6-1.8mg/L. The yeast-expressed VP1 protein self-assembled into virus-like particles and exhibited antigenicity, proving to be suitable for serological assays.