Based on new census surveys recently performed by local government representatives, as well as two complementary and expanded expeditions conducted in 1997 and 2004, we present an updated database of the distribution of known native Metasequoia glyptostroboides trees in an area formed by western Hubei, eastern Chongqing, and northern Hunan in south-central China. The area is located between longitudes 108°20′E (Huangshui Town, Shizhu County, Chongqing) and 109°40′E (Tani Township, Longshan County, Hunan) and 30°10′N (Jiannan Town and Moudao Town, Lichun City, Hubei) and latitudes 29°10′N (Luota Township, Longshan County, Hunan). A total of 5396 native Metasequoia trees exist either as scattered (isolated) individual trees or in populations from two regions of this area. The majority of trees (5391 trees) grow in the first region, which includes eastern Chongqing and western Hubei. Only five trees exist in the second region at western Hunan. The database provides an updated census for the current status of living Metasequoia and a basis for further scientific research and better conservation strategies. Additionally, for the convenience of non-Chinese speakers, we have summarized the historical conservation process of this species and clarified changes in the geographic names and the structure of the Chinese administrative systems.