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Nature Research, Nature Biotechnology, 12(26), p. 1336-1338, 2008

DOI: 10.1038/nbt1208-1336

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Beyond Edman Degradation: Automated De novo Protein Sequencing of Monoclonal Antibodies

Journal article published in 2008 by Nuno Bandeira, Victoria Pham, Pavel Pevzner, David Arnott, Jennie R. Lill
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

De novo protein sequencing of monoclonal antibodies is required when the cDNA or the original cell line is not available, or when characterization of posttranslational modifications is needed to verify antibody integrity and effectiveness. We demonstrate that Comparative Shotgun Protein Sequencing (CSPS) based on tandem mass spectrometry can reduce the time required to sequence an antibody to 72 hours, a dramatic reduction as compared to the classical technique of Edman degradation. We therefore argue that CSPS has the potential to be a disruptive technology for all protein sequencing applications.