Information on both structure and functions is essential to evaluate the ecological integrity of stream ecosystems and their response to natural and anthropogenic disturbance. Leaf-bags have been widely employed to assess stream ecosystem processes and the degree of leaf mass consumption has been proposed as one of the most useful functional descriptor in aquatic environments. However, the breakdown rate of leaves has been compared with structural indicators of macroinvertebrate assemblages derived from leaf-bags or from benthic samples, without any direct comparison on the characteristics of communities sampled with the two methods. The main objective of the paper is to conduct a comparative analysis of the structure, functional organization and biological traits of macroinvertebrate assemblages from artificial leaf packs and from benthic samples of a third-order stream in the Central Apennines (Italy).