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Nature Research, Nature Biotechnology, 7(29), p. 572-573, 2011

DOI: 10.1038/nbt.1910

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Sequencing technology does not eliminate biological variability

Journal article published in 2011 by Kasper D. Hansen ORCID, Zhijin Wu, Rafael A. Irizarry, Jeffrey T. Leek
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

RNA sequencing has generated much excitement for the advantages offered over microarrays. This excitement has led to a barrage of publications discounting the importance of biological variability; as microarray publications did in the 1990s. By comparing microarray and sequencing data, we demonstrate that expression measurements exhibit biological variability across individuals irrespective of measurement technology. Our analysis suggests RNA-sequencing experiments designed to estimate biological variability are more likely to produce reproducible results.