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American Society for Microbiology, Applied and Environmental Microbiology, 3(77), p. 1097-1100, 2011

DOI: 10.1128/aem.01541-10

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Cofactor Trapping, a New Method To Produce Flavin Mononucleotide

Journal article published in 2010 by Ulrich Krauss, Vera Svensson, Astrid Wirtz ORCID, Esther Knieps-Grünhagen, Karl-Erich Jaeger
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli . This, in turn, allows the isolation of highly pure FMN.