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Frontiers Media, Frontiers in Genetics, (06)

DOI: 10.3389/fgene.2015.00139

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Transposon insertion mapping with PIMMS – Pragmatic Insertional Mutation Mapping System

Journal article published in 2015 by Adam M. Blanchard, James A. Leigh, Sharon A. Egan, Richard D. Emes ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has been developed for simple conditionally essential genome discovery experiments in bacteria. Capable of using raw sequence data files alongside a FASTA sequence of the reference genome and GFF file, PIMMS will generate a tabulated output of each coding sequence with corresponding mapped insertions accompanied with normalized results enabling streamlined analysis. This allows for a quick assay of the genome to identify conditionally essential genes on a standard desktop computer prioritizing results for further investigation.