Humana Press, Methods in Molecular Biology, p. 33-44, 2020
DOI: 10.1007/978-1-0716-0621-6_2
Humana Press, Methods in Molecular Biology, p. 81-92
DOI: 10.1007/978-1-61779-008-9_5
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Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of the reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation of enzyme specificity.