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Elsevier, Analytical Biochemistry, 1(90), p. 47-57, 1978
DOI: 10.1016/0003-2697(78)90007-6
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A new and specific assay for ammonia and glutamine sensitive to 100 pmol
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Abstract
A new enzymatic-radiochemical technique of NH4+ determination has been developed that is sensitive and specific. The reaction of α-[1-14C]ketoglutarate with NH4+ yields [1-14C]glutamate as a direct measure of the NH4+ over a range of 0.1 to 10.0 nmol. By the measurement of the NH4+ present in a sample before and after glutamine hydrolysis the assay also allows the determination of glutamine.