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Nature Research, Nature Methods, 12(8), p. 1047-1049, 2011

DOI: 10.1038/nmeth.1744

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Live-cell 3D super-resolution imaging in thick biological samples

Journal article published in 2011 by Francesca Cella Zanacchi ORCID, Zeno Lavagnino, Michela Perrone Donnorso, Alessio Del Bue, Laura Furia, Mario Faretta, Alberto Diaspro
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.