This paper investigates the impact of job related stress on Job Satisfaction of nursing staff working in hospitals. Job stress is one of the most important workplace health risks for employees, and job satisfaction has been considered as a crucial factor in the provision of high quality services and superior performance at hospitals. This paper presents a field survey. Drawing on a sample of 271 nurses operating in Greek hospitals, we examined the degree to which stressors such as conflict, workload, interpersonal relationships, career development, information access and feedback influence job satisfaction aspects such as physical environment, career opportunities, management style, job enrichment, rewards and job security. Results showed that conflict, heavy workload and lack of job autonomy are negatively associated with all job satisfaction dimensions, while shortage in information access and feedback is positively related to employees’ satisfaction with rewards and job security.