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Public Library of Science, PLoS Genetics, 5(9), p. e1003540, 2013

DOI: 10.1371/journal.pgen.1003540

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Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana

Journal article published in 2013 by Hirokazu Tanaka, Saeko Kitakura, Hana Rakusová, Tomohiro Uemura, Mugurel I. Feraru, Ioan Mugurel Feraru, Riet M. U. De Rycke, Stéphanie Robert ORCID, Tatsuo Kakimoto, Jiří Friml
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and mediate intercellular polar transport of the plant hormone auxin that is crucial for a multitude of developmental processes in plants. PIN localization is under extensive control by environmental or developmental cues, but mechanisms regulating PIN localization are not fully understood. Here we show that early endosomal components ARF GEF BEN1 and newly identified Sec1/Munc18 family protein BEN2 are involved in distinct steps of early endosomal trafficking. BEN1 and BEN2 are collectively required for polar PIN localization, for their dynamic repolarization, and consequently for auxin activity gradient formation and auxin-related developmental processes including embryonic patterning, organogenesis, and vasculature venation patterning. These results show that early endosomal trafficking is crucial for cell polarity and auxin-dependent regulation of plant architecture.