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Oxford University Press, Nucleic Acids Research, suppl_2(35), p. W495-W498, 2007

DOI: 10.1093/nar/gkm406

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Sequence harmony: detecting functional specificity from alignments

Journal article published in 2007 by K. Anton Feenstra ORCID, Walter Pirovano, Klaas Krab, Jaap Heringa
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Multiple sequence alignments are often used for the identification of key specificity-determining residues within protein families. We present a web server implementation of the Sequence Harmony (SH) method previously introduced. SH accurately detects subfamily specific positions from a multiple alignment by scoring compositional differences between subfamilies, without imposing conservation. The SH web server allows a quick selection of subtype specific sites from a multiple alignment given a subfamily grouping. In addition, it allows the predicted sites to be directly mapped onto a protein structure and displayed. We demonstrate the use of the SH server using the family of plant mitochondrial alternative oxidases (AOX). In addition, we illustrate the usefulness of combining sequence and structural information by showing that the predicted sites are clustered into a few distinct regions in an AOX homology model. The SH web server can be accessed at www.ibi.vu.nl/programs/seqharmwww.