Published version of an article in the journal: Abstract and Applied Analysis. Also available from the publisher at: http://dx.doi.org/10.1155/2014/303840 Open Access ; State estimation problem is considered for a kind of wireless network control system with stochastic uncertainty and time delay. A sliding mode observer is designed for the system under the situation that no missing measurement occurs and system uncertainty happens in a stochastic way. The observer designed for the system can guarantee the system states will be driven onto the sliding surface under control law, and the sliding motion of system states on sliding surface will be stable. By constructing proper Lyapunov-Krasovskii functional, sufficient conditions are acquired via linear matrix inequality. Finally, simulation result is employed to show the effectiveness of the proposed method.