AbstractBiomass burning (BB) is a major source of pollutants that impact local, regional, and global climate, air quality, and public health. However, the influence of burning conditions and fuel type on the emission factors of pollutants is still not well understood. Here, we present the results from a laboratory study of emission factors (EFs) of pollutants from six different sub‐Saharan African biomass fuels combusted under a wide range of burning conditions, ranging from smoldering to flaming. We found that particulate matter (PM) and carbon monoxide (CO) EFs (g (kg wood)⁻¹) are highly sensitive to the burning conditions, with an order of magnitude variation between flaming and smoldering burning conditions. Nitric oxide (NO) EF shows a fuel type dependence, with higher NO EFs for fuels with larger nitrogen content. While CO is not generally a proxy for PM_2.5 emissions, in this work a correlation was found between CO and PM emissions generated by combustion of seven wood fuels with moisture content (dry basis) <10% in a tube furnace and measured from a laboratory smog chamber with a temperature of ∼21–24°C and an RH below 5%. Unlike total PM, EFs of inorganic particle‐phase species do not show dependence on burning conditions. Finally, we showed that burning biomass fuels in a tube furnace would be a useful experimental approach to study BB emission under controlled burning conditions.