Hypoxia and adverse health outcomes might be affected by O3 pollution in the highland city of Lhasa. NOx emissions can amplify the role of volatile organic compounds (VOCs) in the secondary production of O3 under the conditions of high ultraviolet (UV) radiation levels and unfavourable dispersion patterns in the Lhasa River valley. Here, online C2–C11 VOC measurements, accompanied by other parameters concerning the O3 chemical budget, were first obtained and employed to identify the key VOC species and key sources of VOCs in terms of the loss rate against OH radicals (LOH), ozone formation potential (OFP), secondary organic aerosol potential (SOAP), and toxicity. Oxygenated VOCs (OVOCs) not only were the most abundant VOCs but also dominated LOH, OFP, and toxicity. Isoprene and anthropogenic VOCs were further identified as precursors of these OVOCs. Aromatics accounted for 5 % of the total VOCs (TVOCs) but contributed 88 % to the SOAP and 10 % to the toxicity. As the primary oxidative intermediates of aromatics were not well characterized by our measurements, the environmental impact of aromatics could be underestimated by our data. Source appointment and ternary analysis of benzene, toluene, and ethylbenzene confirmed the combined contribution of traffic emissions, solvent usage, and biomass burning. This suggests that O3 precursors are mainly from sources associated with residents' lives, except for solvent usage emissions which contribute to aromatics. Preliminary comparisons between source spectrums of transport sector emissions with positive matrix factorization (PMF)-decomposed ones and our measured ones suggest that vehicle emission patterns of VOCs at high altitude generally follow the ground-level impression. More quantitative data are required to further confirm this point though. Emission reduction strategy analysis for O3 pollution control highlighted multiple benefits of the simultaneous reduction in NOx originating from diesel vehicle emissions, biomass burning, and background sources (possibly dominated by incense burning). The notable biogenic emission contribution to the OFP was also first confirmed in our study, and this highlights the side effects of the government's pursuit of a greener city.