<b><i>Introduction:</i></b> Examination of subungual pigmented lesions is sometimes a diagnostic challenge for clinicians. <b><i>Objectives:</i></b> The study was aimed to investigate characteristic patterns in optical coherence tomography (OCT) of subungual hematomas and determine distinctive features that can differentiate them from subungual melanocytic lesions. <b><i>Methods:</i></b> VivoSight® (Michelson Diagnostics, Maidstone, UK) was used to examine 71 subungual hematomas and 11 subungual melanocytic lesions in 69 patients (18 female and 51 male patients). <b><i>Results:</i></b> On OCT, bleeding was related to sharply defined black sickle-shaped (<i>p</i> &#x3c; 0.001) or globular regions (not significant [ns]) with a hyperreflective margin (0.002), a grey center (0.013), hyperreflective lines in the area (ns) or periphery (<i>p</i> = 0.031), peripheral fading (<i>p</i> = 0.029), and red dots in the area (<i>p</i> = 0.001). In the 1 case of melanoma in situ examined, we found curved vessels with irregular sizes and distribution on the dermis of the nailbed, while subungual hematomas and subungual benign nevi presented as clustered red dots and/or regularly distributed curved vessels. <b><i>Conclusion:</i></b> Our findings indicate that the use of OCT in addition to dermoscopy provides high-resolution optical imaging information for the diagnosis of subungual hematoma and facilitates the differential diagnosis of subungual hematomas and subungual melanocytic lesions.