Published in
American Society for Microbiology, Applied and Environmental Microbiology, 6(88), 2022
DOI: 10.1128/aem.02187-21
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Mechanism of Cooperative Degradation of Gum Arabic Arabinogalactan Protein by Bifidobacterium longum Surface Enzymes
Journal article published in 2022 by
Yuki Sasaki,
Masahiro Komeno,
Akihiro Ishiwata 
,
Ayako Horigome,
Toshitaka Odamaki ,
Jin-Zhong Xiao,
Katsunori Tanaka,
Yukishige Ito,
Kanefumi Kitahara,
Hisashi Ashida,
Kiyotaka Fujita
,
Ayako Horigome,
Toshitaka Odamaki ,
Jin-Zhong Xiao,
Katsunori Tanaka,
Yukishige Ito,
Kanefumi Kitahara,
Hisashi Ashida,
Kiyotaka Fujita
This paper is made freely available by the publisher.
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Abstract
Terminal galactose residues of the side chain of gum arabic arabinogalactan protein (AGP) are mainly substituted by α1,3/α1,4-linked Ara f and β1,6-linked α- l -Rha p -(1→4)-β- d -Glc p A residues. This study found a multidomain BlArafE with GH43_22 and GH43_34 catalytic domains showing cooperative action for degrading α1,3/α1,4-linked Ara f of the side chain of gum arabic AGP.