Soil column tests were conducted to investigate the effects of grass type on water infiltration in a three-layer landfill cover under drying and wetting conditions. Five soil columns were prepared, including one bare, two Bermuda grass-planted and the other two vetiver-planted. During the drying period, the suction of vetiver-planted soil column was the largest, while that of bare case was the lowest. During the wetting period, the infiltration rate shows a bimodal form due to the contrasting hydraulic properties of different soil layers. The infiltration rate of vetiver-planted soil column was the lowest, followed by Bermuda grass-planted and bare cases. Correspondingly, the vetiver-planted soil column retained the maximum suction and the deepest ponding depth during rainfall. This was likely due to the larger leaf area and deeper roots of vetiver than those of Bermuda grass, thus inducing the maximum initial suction by root water uptake before rainfall and reducing the water permeability by root occupations of soil pores. These results show that vetiver is more effective than Bermuda grass to reduce water percolation through the three-layer landfill cover.