The reason for this study was to compare the morphology of mites from three Japanese samples with topotypes from the Nalepa collection, in order to correctly identify them. With this purpose eriophyoid specimens were recovered from five vials of the Nalepa mite collection in the Natural History Museum of Vienna. We identified seven species and a new combination is established for one of them - Aceria thomasi and Aculops thymi, from two vials labeled Thymus serpyllum (Lamiaceae); Eriophyes spiraeae from a vial labeled Spiraea crenifolia (Rosaceae); and Peralox longirostris n. comb., Shevtchenkella ulmi, Aceria filiformis and Aculus mastigophorus from two vials labeled Ulmus campestris (Ulmaceae). A supplementary description is presented for these species. The Japanese samples were collected from ‘Shiso’, Perilla frutescens var. crispa (Lamiaceae), from Spiraea thunbergia (Rosaceae) and from Juglans mandshurica (Juglandaceae). The mites collected from ‘Shiso’ were identified as A. thymi, but the other two Japanese samples did not match the topotypes. These two samples may be new species and require further taxonomic studies.