This is an Open Access article published in Atmospheric Measurement Techniques Discussions, published by Copernicus Publications on behalf of European Geosciences Union, copyright The Author(s). ; This study validates trace gas and thermodynamic retrievals from nadir infrared spectroscopic measurements recorded by the UK Met Office Airborne Research Interferometer Evaluation System (ARIES) – a thermal infrared, Fourier transform spectrometer (TIR-FTS) on the UK Facility for Airborne Atmospheric Measurements (FAAM) BAe-146 aircraft. Trace-gas-concentration and thermodynamic profiles have been retrieved and validated for this study throughout the troposphere and planetary boundary layer (PBL) over a range of environmental variability using data from aircraft campaigns over and around London, the US Gulf Coast, and the Arctic Circle during the Clear air for London (ClearfLo), Joint Airborne IASI (Infrared Atmospheric Sounding Interferometer) Validation Experiment (JAIVEx), and Measurements, process studies, and Modelling (MAMM) aircraft campaigns, respectively. Vertically resolved retrievals of temperature and water vapour (H2O), and partial-column retrievals of methane (CH4), carbon monoxide (CO), and ozone (O3) (over both land and sea) were compared to corresponding measurements from high-precision in situ analysers and dropsondes operated on the FAAM aircraft. Average degrees of freedom for signal (DOFS) over a 0–9 km column range were found to be 4.97, 3.11, 0.91, 1.10, and 1.62 for temperature, H2O, CH4, CO, and O3, respectively, when retrieved on 10 vertical levels. Partial-column mean biases (and bias standard error) between the surface and ~ 9 km, when averaged across all flight campaigns, were found to be −0.7(±0.3) K, −479(±56) ppm, −11(±2) ppb, −3.3(±1.0) ppb, and +3.5(±1.0) ppb, respectively, whilst the typical a posteriori (total) uncertainties for individually retrieved profiles were 0.4, 9.5, 5.0, 21.2, and 15.0 %, respectively. Averaging kernels (AKs) derived for progressively lower altitudes show improving sensitivity to lower atmospheric layers when flying at lower altitudes. Temperature and H2O display significant vertically resolved sensitivity throughout the column, whilst trace gases are usefully retrieved only as partial-column quantities, with maximal sensitivity for trace gases other than H2O within a layer 1 and 2 km below the aircraft. This study demonstrates the valuable atmospheric composition information content that can be obtained by ARIES nadir TIR remote sensing for atmospheric process studies.