AbstractOnce the age and metallicity are fixed, the colour distribution of horizontal branch stars in a globular cluster depends on few parameters: the helium abundance of the population and the mass lost during the pre-HB stages. These parameters are usually derived from the HB itself, hence they are degenerate. Breaking this degeneracy and understanding their role is a tricky and challenging problem that no study has solved yet. Combining the information obtained from the chromosome maps and the analysis of multi-band photometry with state of the art stellar evolution models, we can obtain a solid estimate of Y for the various stellar populations in a GC. We will then have, for the first time, the possibility to break the parameters’ degeneracy on the HB, understand the role of the mass loss, and lay the foundation to build another piece of the multiple populations mosaic.