Published in
Elsevier, Journal of Biotechnology, (182-183), p. 97-103, 2014
DOI: 10.1016/j.jbiotec.2014.04.014
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- [www.ncbi.nlm.nih.gov] | PDF
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Reduced quenching and extraction time for mammalian cells using filtration and syringe extraction
Journal article published in 2014 by
Juan A. Hernández Bort,
Vinoth Shanmukam,
Martin Pabst 
,
Markus Windwarder,
Laura Neumann,
Ali Alchalabi,
Guido Krebiehl,
Gunda Koellensperger,
Stephan Hann ,
Denise Sonntag,
Friedrich Altmann,
Christine Heel,
Nicole Borth
,
Markus Windwarder,
Laura Neumann,
Ali Alchalabi,
Guido Krebiehl,
Gunda Koellensperger,
Stephan Hann ,
Denise Sonntag,
Friedrich Altmann,
Christine Heel,
Nicole Borth
This paper is available in a repository.
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Abstract
•Fast quenching method enabling transfer of washed cells into liquid nitrogen within 15 s.•Optimized extraction protocol for full recovery of extract solution from cells on the filter.•Enables accurate analysis of cellular metabolites and improved maintenance for instance of the energy charge of cells.