To combat the rutting effect and other distresses in asphalt concrete pavement, certain modifiers and additives have been developed to modify the asphalt mixture to improve its performance. Although few additives exist, nanomaterials have recently attracted significant attention from the pavement industry. Several experimental studies have shown that the use of nanomaterials to modify asphalt binder results in an improved oxidative aging property, increased resistance to the rutting effect, and improves the rheological properties of the asphalt mixture. However, despite the numerous benefits of using nanomaterials in asphalt binders and materials, there are various uncertainties regarding the environmental impacts of nano-modified asphalt mixtures (NMAM). Therefore, this study assessed a Nano-Silica-Modified Asphalt Mixtures in terms of materials production emissions through the Life Cycle Assessment methodology (LCA), and the results were compared to a conventional asphalt mixture to understand the impact contribution of nano-silica in asphalt mixtures. To be able to compare the relative significance of each impact category, the normalized score for each impact category was calculated using the impact scores and the normalization factors. The results showed that NMAM had a global warming potential of 7.44563 × 103 kg CO2-Eq per functional unit (FU) compared to 7.41900 × 103 kg CO2-Eq per functional unit of the conventional asphalt mixture. The application of LCA to NMAM has the potential to guide decision-makers on the selection of pavement modification additives to realize the benefits of using nanomaterials in pavements while avoiding potential environmental risks.