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Public Library of Science, PLoS ONE, 4(8), p. e62054, 2013

DOI: 10.1371/journal.pone.0062054

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A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs

Journal article published in 2013 by Karamjit Singh Dolt, Melanie L. Lawrence, Eve Miller-Hodges, Joan Slight, Anna Thornburn, Paul S. Devenney, Peter Hohenstein ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models.