Context: The prevalence of sexual dysfunction, low vitality and poor physical function increases with aging, as does the prevalence of low total and free testosterone (TT and FT) levels. However, the relationship between sex hormones and age-related alterations in older men is not clear. Objective: To test the hypotheses that baseline serum TT, FT, estradiol (E2) and sex hormone-binding globulin (SHBG) levels are independently associated with sexual function, vitality and physical function in older symptomatic men with low testosterone levels participating in the Testosterone Trials (TTrials). Design: Cross-sectional study of baseline measures in the TTrials. Setting: 12 sites in the United States. Participants: The 788 TTrials participants were ≥65 years and had evidence of sexual dysfunction, diminished vitality and/or mobility disability, and an average of two TT <275 ng/dL. Interventions: None. Main Outcome Measures: Question 4 of Psychosocial Daily Questionnaire (PDQ-Q4), the FACIT-Fatigue Scale and the 6-minute walk test. Results: Baseline serum TT and FT, but not E2 or SHBG levels had small, but statistically significant associations with validated measures of sexual desire, erectile function and sexual activity. None of these hormones was significantly associated within or across trials with FACIT-Fatigue, PHQ-9 Depression or Physical Function-10 scores, or gait speed. Conclusions: FT and TT levels were consistently, independently and positively associated, albeit to a small degree, with measures of sexual desire, erectile function and sexual activity, but not with measures of vitality or physical function in symptomatic older men with low T who qualified for the TTrials.