Published in

Elsevier, Journal of Biological Chemistry, 14(285), p. 10519-10526, 2010

DOI: 10.1074/jbc.m109.068890

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Identification of Specific Lipid-binding Sites in Integral Membrane Proteins*

Journal article published in 2010 by Marc F. Lensink ORCID, Cédric Govaerts, Jean-Marie Ruysschaert
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Protein-lipid interactions are increasingly recognized as central to the structure and function of membrane proteins. However, with the exception of simplified models, specific protein-lipid interactions are particularly difficult to highlight experimentally. Here, we used molecular dynamics simulations to identify a specific protein-lipid interaction in lactose permease, a prototypical model for transmembrane proteins. The interactions can be correlated with the functional dependence of the protein to specific lipid species. The technique is simple and widely applicable to other membrane proteins, and a variety of lipid matrices can be used.