Deteriorated aerobic response to moderate exercise might precede the manifestation of clinical symptoms of noncommunicable diseases. The purpose of the current study was to verify that the use of current wearable technologies for analysis of pulmonary oxygen uptake (V̇o₂) dynamics during a pseudorandom ternary sequence (PRTS) over-ground walking protocol is a suitable procedure for the investigation of the aerobic response in more realistic settings. A wearable accelerometer located at the hip assessed the magnitude of the input changes delivered to the aerobic system. Eight adults (24 ± 4 yr old, 174 ± 7 cm, and 71.4 ± 7.4 kg) performed two identical PRTS over-ground walking protocols. In addition, they performed on the cycle ergometer two identical pseudorandom binary sequence (PRBS) protocols and one incremental protocol for maximal V̇o₂ determination. In the frequency domain, mean normalized gain amplitude (MNG in %) quantified V̇o₂ dynamics. The MNG during PRTS was correlated ( r = −0.80, P = 0.01) with the V̇o₂ time constant (τ) obtained during cycling. The MNG estimated during PRBS was similar to the MNG estimated during PRTS ( r = 0.80, P = 0.01). The maximal V̇o₂ correlated with the MNG obtained during the PRBS ( r = 0.79, P = 0.01) and PRTS ( r = 0.78, P = 0.02) protocols. In conclusion, PRTS over-ground walking protocol can be used to evaluate the aerobic system dynamics by the simultaneous measurement of V̇o₂ and hip acceleration. In addition, the aerobic response dynamics from PRBS and PRTS were correlated to maximal V̇o₂. This study has shown that wearable technologies in combination with assessment of MNG, a novel indicator of system dynamics, open new possibilities to monitor cardiorespiratory health under conditions that better simulate activities of daily living than cardiopulmonary exercise testing performed in a medical environment.