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American Society for Microbiology, Journal of Virology, 18(88), p. 10970-10974, 2014

DOI: 10.1128/jvi.00745-14

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The Linker Region of NS3 Plays a Critical Role in the Replication and Infectivity of Hepatitis C Virus

Journal article published in 2014 by Andrew Kohlway, Nathan Pirakitikulr, Steve C. Ding, Feng Yang, Dahai Luo ORCID, Brett D. Lindenbach, Anna M. Pyle
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro . Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3-4A.